FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838682

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838682
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10874879
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCG48150.045.47454145
CTGAACG59550.040.5434145
GAGCACC173400.025.045828145
CACACGG101500.024.858269145
GATCGGG33200.022.711874145
AAGAGCC166650.021.40511145
CGGAAGG50250.020.921347145
CCAGTCC135650.018.974321145
CAGAATG234950.018.206825145
ACACGTA35400.014.541619145
AACTCCC132000.013.347196145
CGGAAGC141550.013.317453145
CGGAACG10505.11136E-1011.738627145
TCGCGTT15500.011.2263155145
CATGTCG20350.011.044726145
CTCCAGG246850.09.986274145
CGTCTGG45050.09.817315145
GGGGGGG6035750.09.667505145
GGCGTCG18150.09.587212145
ATCGGAG30250.09.34753145
Produced by FastQC (version 0.11.5)