FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838683

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838683
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10874879
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG102200.040.785023145
CCGTATA63450.027.533993145
CGGTGGG195600.023.978344145
TCTCGGG168600.020.895967145
CGCCGTT35750.019.263329145
GCCGTAA47550.016.617273145
TCGCCGG209050.015.95113145
CGGAAGC145950.015.397184145
CCGGATA28100.014.18862145
AGAGCGG340850.014.164287145
GTAGATA259250.013.170004145
GAGTGTT189950.012.975478145
TCGTGTT225050.012.819994145
TCGCGGG29150.012.682808145
TCGGAAC25200.012.657149145
GGGAAAA226400.012.551435145
TATCGGG25650.012.435094145
GTAGGGG121900.012.190848145
TAGATCA143450.011.218536145
CGTCGTT15600.011.152453145
Produced by FastQC (version 0.11.5)