FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838709

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838709
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69713221
Sequences flagged as poor quality0
Sequence length28
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGGA429050.018.481545
CCCGGAA183500.018.3216781
GGCACGT231700.018.215011
AAGAGCC190500.018.0783637
TTAGGAG370700.018.046586
TAAGAGC185900.018.0168536
ACGTTAG477100.017.9856533
CTGGCAG259650.017.9496171
CACGTTA384800.017.9337582
CCACGTT465150.017.6922281
AGGGAAG152900.017.6327973
GCCCGAA132750.017.3758811
TAGGAGT390250.017.3088137
AATGCGG146600.017.24955610
AGAAGGG147950.017.2333438
GAGTCTG399000.016.97892810
GATAGCA269350.016.9432815
GTAAGAG210950.016.9358715
CGTTAGG460250.016.8080714
AGGAGTC405900.016.7552628
Produced by FastQC (version 0.11.5)