FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838722

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838722
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38466099
Sequences flagged as poor quality0
Sequence length94
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC435770.11328676713487375No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG412730.10729707735634955No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT4153400.081.420811
AGCAGTG4644450.072.8003852
CAGTGGT5988100.056.3592224
GCAGTGG6050500.055.959773
AGTGGTA6268800.053.7899865
GTGGTAT6691800.050.3115926
TGGTATC6688800.050.228917
GGTATCA6854150.049.0210348
GTATCAA6936550.048.4400839
ACGACGC31750.032.703835
CACGACG36200.028.9266914
CGACGCT36850.028.2970546
ATCAACG6981050.027.23064610-11
CAACGCA7039000.027.02082412-13
AGAGTAC7172150.026.19578718-19
GCAGAGT7275100.026.00497616-17
AGTACAT7210800.025.81848120-21
TACATGG7169900.025.79746622-23
ACGCAGA7441550.025.63128714-15
TACACGA42100.025.083772
Produced by FastQC (version 0.11.5)