FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838733

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838733
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48825770
Sequences flagged as poor quality0
Sequence length28
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCGAA85050.017.0938591
GTGGAGA107550.016.9662461
TACCGGG136050.016.9552571
CGGACAC96850.016.863431
AAACGAA129250.016.629661
GGGCTAC88350.016.4304681
AGTGCGG100350.016.417322
CCCGGAA74350.016.3417951
CGGAACC78550.016.0704821
TAACGAC87200.016.0665861
ACGCACG106850.016.0164971
TGCGGAG74400.015.789973
GGAGGAT145450.015.7611241
AGGATAG87850.015.663853
AACCTGA101600.015.6309111
AGAAAAG62400.015.5301013
GGGATAG62250.015.355483
CGGGAAG45350.015.256553
GCGACAA84500.015.2560110
TGGGCTG111350.015.2407311
Produced by FastQC (version 0.11.5)