FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838735

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838735
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48727232
Sequences flagged as poor quality0
Sequence length28
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCGAA80800.017.4469221
CGGACAC90700.017.180551
GTGGAGA109500.016.9744851
TACCGGG132550.016.5216881
GGGCTAC89550.016.5040871
AGTGCGG98650.016.4868932
AAACGAA128050.016.3975851
TGCGGAG76500.016.319973
TAACGAC82900.016.274851
CGGAACC79400.016.21611
CCCGGAA72850.016.148391
ACGCACG105300.016.0420951
GGAGGAT146950.016.0260051
AACCTGA103200.015.9419911
AGGATAG88550.015.9376043
TGGGCTG104950.015.5503361
GGAGAAC81900.015.5061141
GCACGAG100350.015.47755053
CTGGACG58950.015.4757541
ATACCGA88350.015.3954721
Produced by FastQC (version 0.11.5)