FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838738

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838738
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44329371
Sequences flagged as poor quality0
Sequence length94
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG494850.11163027781287489No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG444400.10024956140252926No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT4830350.081.4872061
AGCAGTG5406750.072.742622
CAGTGGT6962700.056.4044384
GCAGTGG7135250.055.1859473
AGTGGTA7258850.054.0777635
TGGTATC7760300.050.44287
GTGGTAT7773550.050.4270066
GGTATCA7959450.049.2050138
GTATCAA8057000.048.6230289
ACGACGC29200.033.600925
CACGACG35550.027.7228364
ATCAACG8104150.027.32249510-11
CGACGCT36050.027.2162826
CAACGCA8176800.027.0891412-13
AGAGTAC8328550.026.2287218-19
GCAGAGT8442000.026.07428616-17
AGTACAT8349500.025.90814620-21
TACATGG8304900.025.8401522-23
ACGCAGA8590000.025.82733214-15
CATGGGA3923300.025.01538724-25
Produced by FastQC (version 0.11.5)