FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838744

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838744
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44349289
Sequences flagged as poor quality0
Sequence length94
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG486050.1095958945362123No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG443590.10002189663063145No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT4855750.081.551491
AGCAGTG5439600.072.748372
CAGTGGT7012800.056.3492284
GCAGTGG7199500.055.034633
AGTGGTA7293200.054.1369325
TGGTATC7792100.050.533537
GTGGTAT7808750.050.5125546
GGTATCA7988400.049.3088388
GTATCAA8089400.048.7038659
ACGACGC31500.035.058535
CGACGCT35350.031.1158126
CACGACG37050.029.2130854
ATCAACG8121600.027.42212510-11
CAACGCA8195800.027.19966912-13
AGAGTAC8334400.026.37374918-19
GCAGAGT8454300.026.2091816-17
AGTACAT8366400.026.0057720-21
ACGCAGA8602050.025.95660614-15
TACATGG8310950.025.95232622-23
CATGGGA3949150.024.93870724-25
Produced by FastQC (version 0.11.5)