FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838750

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838750
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44849223
Sequences flagged as poor quality0
Sequence length94
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG667670.1488699146471278No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC655070.14606050142719307No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT5276000.082.669161
AGCAGTG5925650.073.580992
CAGTGGT7598350.057.3330844
GCAGTGG7701800.056.6829533
AGTGGTA7953850.054.7456745
TGGTATC8300050.052.326317
GTGGTAT8319750.052.251256
GGTATCA8463900.051.3221138
GTATCAA8538700.050.8831679
ACGACGC32800.030.4482575
CGACGCT35000.028.7857746
ATCAACG8530200.028.6808910-11
CAACGCA8572600.028.55093212-13
AGAGTAC8661800.027.93866318-19
GCAGAGT8788150.027.7056916-17
TACATGG8673500.027.57970222-23
AGTACAT8728750.027.5540920-21
ACGCAGA9005750.027.21253414-15
CATGGGA4039000.026.61922624-25
CACGACG37900.026.2349154
Produced by FastQC (version 0.11.5)