FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838827

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838827
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49945734
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT2087530.41795961993470754No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG580330.11619210561606723No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA550540.11022763225383775No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT393950.025.32121545
GACCGCG448150.022.7180925
AGACCGC450150.022.6469614
ACCGCGT448800.022.6400896
CCGCGTT458550.022.143987
CAGACCG461850.022.053743
CGCGTTC468750.021.6669278
AGAACGA469200.021.29338344
TCAGACC495700.020.765622
GCGTTCT506450.020.1251329
AATACGG511950.019.61426532
AACGCTA187550.019.54234723
ATACGGA514500.019.50830733
ACGCTAC189850.019.30559724
CAATACG527100.019.1401531
AAGAACG522850.019.09537143
TACGGAG526700.019.03934734
TTCGACG24300.018.9812436
CTCAGAC578300.018.9419351
CGCTACC196100.018.69029625
Produced by FastQC (version 0.11.5)