FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838834

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838834
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39799237
Sequences flagged as poor quality0
Sequence length94
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC541320.1360126577300967No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA510730.12832658073319347No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG434330.10913023282330765No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT5619850.083.05331
AGCAGTG6255100.074.583382
GCAGTGG7995650.058.4374963
CAGTGGT8065200.057.7933584
AGTGGTA8361000.055.7181935
GTGGTAT9127950.050.9590266
TGGTATC9148600.050.762737
GGTATCA9407850.049.407368
GTATCAA9499600.048.925329
ACGACGC35850.031.1726385
TACACGA40600.027.5278072
ATCAACG9599450.027.08554810-11
CAACGCA9679500.026.9013512-13
CACGACG42100.026.544874
AGAGTAC9791150.026.23044618-19
ACGCAGA9985850.026.10516414-15
GCAGAGT9917000.026.09433616-17
AGTACAT9797250.025.93703520-21
TACATGG9737150.025.92690522-23
CATGGGA4529750.025.36766224-25
Produced by FastQC (version 0.11.5)