FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838838

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838838
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36605899
Sequences flagged as poor quality0
Sequence length94
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC491830.13435812626811872No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA488040.13332277401519355No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT5192000.082.914351
AGCAGTG5748050.074.848542
GCAGTGG7369650.058.4328163
CAGTGGT7427300.057.831764
AGTGGTA7718300.055.6200035
GTGGTAT8436800.050.8222436
TGGTATC8454300.050.663447
GGTATCA8688150.049.3088958
GTATCAA8777600.048.8126569
ACGACGC33950.029.9367145
CACGACG36200.028.1975484
ATCAACG8871850.026.97038310-11
CAACGCA8943350.026.77862712-13
TACACGA38900.026.4684812
AGAGTAC9054250.026.10574718-19
ACGCAGA9228300.025.97989814-15
GCAGAGT9173900.025.95134516-17
AGTACAT9063750.025.82353820-21
TACATGG9007150.025.80385422-23
CATGGGA4196750.025.184524-25
Produced by FastQC (version 0.11.5)