FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838840

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838840
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44910173
Sequences flagged as poor quality0
Sequence length94
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC612040.13628092681807305No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA594470.13236867290624776No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT6408550.082.911681
AGCAGTG7120950.074.571252
GCAGTGG9090100.058.450723
CAGTGGT9148900.057.9428064
AGTGGTA9492750.055.837965
GTGGTAT10363100.051.1008266
TGGTATC10386100.050.90767
GGTATCA10674050.049.5495348
GTATCAA10779350.049.071169
ACGACGC40500.031.8305745
CACGACG45550.028.108424
ATCAACG10886550.027.17531410-11
CAACGCA10975250.026.97573912-13
TACACGA47850.026.8512292
AGAGTAC11111150.026.31818618-19
ACGCAGA11315300.026.20258714-15
GCAGAGT11251350.026.16288616-17
AGTACAT11124300.026.03574220-21
TACATGG11070850.026.0027222-23
CATGGGA5162200.025.4281824-25
Produced by FastQC (version 0.11.5)