FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838842

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838842
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45476106
Sequences flagged as poor quality0
Sequence length94
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC629200.13835837219659924No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA567800.12485677643551979No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT6421000.082.9455261
AGCAGTG7151150.074.450272
GCAGTGG9161700.058.174033
CAGTGGT9238950.057.588094
AGTGGTA9585100.055.4868245
GTGGTAT10470200.050.7374046
TGGTATC10485250.050.575627
GGTATCA10778800.049.2198758
GTATCAA10887950.048.727549
ACGACGC39700.029.9225185
TACACGA43800.027.6272032
ATCAACG10997850.027.04651810-11
CAACGCA11081450.026.86274712-13
AGAGTAC11225250.026.20782918-19
ACGCAGA11429650.026.07525814-15
GCAGAGT11360200.026.06104316-17
AGTACAT11245950.025.91650820-21
TACATGG11172250.025.90079322-23
CATGGGA5195050.025.38212624-25
CACGACG48400.024.8165914
Produced by FastQC (version 0.11.5)