FastQCFastQC Report
Fri 27 May 2022
EGAF00004838862

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838862
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101840189
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATT9605460.9431895300194307Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGCGACGACCCATTCGA5640950.5539021535005203Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCAT4940060.4850796182241964Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT2599350.25523813589937466Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATTCG2549620.25035499492248586Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA1608140.157908190842026Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGGGCGGCGACGACCCATTCGAAC1496880.14698323075578737Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTGAGTGTCCCGCGGGGCC1496570.14695279090654476Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATTCGAAC1488890.14619866818982435No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATTC1420290.1394626241316186Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGC1345340.13210305412924953No Hit
GCAGTGGTATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCATTC1313890.1290148823270546Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1218590.11965708351150055No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTCGCCGGCCGAGGTGGGA1126480.11061252056395929Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATTCGA1050640.10316555873634524Clontech SMARTer II A Oligonucleotide (100% over 22bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)