FastQCFastQC Report
Fri 1 Jul 2022
EGAF00004838866

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838866
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120208625
Sequences flagged as poor quality0
Sequence length91
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCAT39626623.2964872528905476Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGCCGCCTGGATACCGCAG12636031.0511749884835635Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATT11347130.9439530649319048Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCATTCGAA10740410.8934808130448211No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAGCGTTTACTTTGAAAAA10349180.8609348954785899Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCCCCCGTGGCGGCGACGA9586980.7975284635357904Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCCCGGCCGGGGGGCGGG9572370.7963130765367294Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGCGACGACCCATTCGA7916660.6585767036267156Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC7048320.5863406223971034Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTCGCCGGCCGAGGTGGGA6879700.5723133427405895Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCCCGGCCGGGGGGCGGGC6757810.5621734713295323Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCATTCGAACG5920550.49252289509176234No Hit
CAGTGGTATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCATTCG5642380.46938229265994846Clontech SMARTer II A Oligonucleotide (100% over 22bp)
GCAGTGGTATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCATTC4983870.41460169767352384Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGCCGATCGCACGCCCCC4812850.4003747651218871Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT4748520.39502323564552877Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGTGGCGGCGACGACCCATTC4226590.3516045541657265Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTACAGTGAAACTGCGAAT4006510.33329638368295117Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGGCGGGCGCCGGCGGC3978040.3309280012145551Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC3952110.3287709180601642Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGGCGACGACCCATTCGAA3771480.3137445420409725Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGGAGCCGCCTGGATACCGCAGCTAGG3743400.3114086031680339No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGCGGGCGCCGGCGGCT3732170.3104743939962711Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCATTCGAACGTCT3476600.2892138563268651No Hit
GTGGTATCAACGCAGAGTACATGGGGGCCCCGGCCGGGGGGCGGGCGCCG3274820.27242803916940234No Hit
AGTGGTATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCATTCGA3180490.2645808485040071Clontech SMARTer II A Oligonucleotide (100% over 21bp)
GTGGTATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATTCGAAC3080300.2562461720196866No Hit
GTGGTATCAACGCAGAGTACATGGGGCCCCCCGTGGCGGCGACGACCCAT3021210.25133055136434673No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGGCGCCGGCGGCTTTG2920970.24299171544471124Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTGAGTGTCCCGCGGGGCCCG2888630.24030139268292938Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGGGGCGGGCGCCGG2815630.23422861712293938Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCCCCTCTCCGGCCCCGGC2767250.23020394751208575Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCAGTGGTATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCATT2672750.2223426147666193Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCTGAGTGTCCCGCGGGGC2669850.22210136751834572Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGCTTTGGTGACTCTAG2577080.21438395123478038Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGCGGCGCCTCGCCTCGGC2564640.213349083728393Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAGGGACTTAATCAACGCA2549970.21212870540695394Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGGAAGCGTTTACTTTGAAAAAATTAG2487000.20689031257116533No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGCCGGCGGCTTTGGTGA2457810.20446203423423237Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCTCCTACTTGGATAACTGTG2311110.19225825101984156Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGGGGGGGATGCGTGCATT2299840.19132071429982667Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCTTTGGTGACTCTAGATA2298530.19121173709457204Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGGCCCCGGCCGGGGGGCGGGCGCCGG2293920.19082823715852335No Hit
GTGGTATCAACGCAGAGTACATGGGGGCGGCGACGACCCATTCGAACGTC2227430.1852970200765544No Hit
GGTATCAACGCAGAGTACATGGGGAGCCGCCTGGATACCGCAGCTAGGAA2223730.18498922186323985No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATCTCAACCTATTCTCAAA2174120.1808622301436357Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATTCCTCGTTCATGGGGAA2139630.1779930516633062Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGACCCCCGCCACGCAGTTTTA2101390.17481191553434705Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGGGGCGGGCGCCGGC2054080.17087625783923574Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGGCTCGCCGGCCGAGGTGGGATCCCG1983040.1649665321435962No Hit
GGTATCAACGCAGAGTACATGGGGGCCCCGGCCGGGGGGCGGGCGCCGGC1975560.16434428062046297No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA1912970.15913749949306882Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGTGTCCCGCGGGGCCCGA1816460.15110895744793687Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGGCCCCCCGTGGCGGCGACGACCCATTC1814710.15096337721190972No Hit
CAGTGGTATCAACGCAGAGTACATGGGGAGCCGCCTGGATACCGCAGCTA1784050.14841281147671392Clontech SMARTer II A Oligonucleotide (100% over 22bp)
GGTATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATTCGAACGT1729950.14391230246581724No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCCGCCTGGATACCGCAGC1695630.14105726606555893Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGGAGCCGCCTGGATACCGCAGCT1614140.1342782183890715Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CAGTGGTATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATTCGA1601280.13320841162603764Clontech SMARTer II A Oligonucleotide (100% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCGTTTACTTTGAAAAAA1596020.13277083903089315Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGGGCCCCGGCCGGGGGGCGGGCGC1582970.1316852264136621Clontech SMARTer II A Oligonucleotide (100% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGCCGGGGGGCGGGCGCCG1570730.13066699664853498Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCCGGTCG1560010.12977521371698578No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCGCCGGCGGCTTTGGTG1553930.12926942638267427Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCGCGCGGCGCCTCGCCTCGG1538340.12797251445143806Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGGCCCCCCGTGGCGGCGACGACCC1530800.1273452716059268Clontech SMARTer II A Oligonucleotide (100% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTGGTGACTCTAGATAA1523970.12677709274188936Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCGAAGCGTTTACTTTGAAAA1519380.12639525657996672Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGAACTGAGGCCATGATTA1518810.126347839017375Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGGGCTCTCGCTTCTGGC1460530.12149960121413918Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGGGCGGGCGCCGGCGG1439500.11975014272062424Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATTCG1436690.11951638245591778Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GGTATCAACGCAGAGTACATGGGGAAGCGTTTACTTTGAAAAAATTAGAG1435070.1193816167517098No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGGCTTTGGTGACTCTAGA1393470.11592096656957852Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCAACGCAGAGTACATGGGGGCCCCGGCCGGGGGGCGGGCGCCGGCGGC1387390.11541517923526702No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGCCCCCCGTGGCGGCGACGACC1383590.11509906215132235Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GTGGTATCAACGCAGAGTACATGGGCGGGCCGATCGCACGCCCCCCGTGG1380990.11488277151493914No Hit
GGTATCAACGCAGAGTACATGGGGCCCCGGCCGGGGGGCGGGCGCCGGCG1368300.1138271068319765No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGGCCCCGGCCGGGGGGCGGGCG1362570.11335043554487044Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GTGGTATCAACGCAGAGTACATGGGGGGGGGCGGGCGCCGGCGGCTTTGG1343400.1117557080450758No Hit
CAGTGGTATCAACGCAGAGTACATGGGGAAGCGTTTACTTTGAAAAAATT1312080.1091502377637212Clontech SMARTer II A Oligonucleotide (100% over 22bp)
ATCAACGCAGAGTACATGGGGAGCCGCCTGGATACCGCAGCTAGGAATAA1310440.10901380828538718No Hit
GGTATCAACGCAGAGTACATGGGGGCGGCGACGACCCATTCGAACGTCTG1273180.10591419708860325No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1240480.10319392639255294No Hit
GTGGTATCAACGCAGAGTACATGGGGGGGGCGGGCGCCGGCGGCTTTGGT1236020.10282290476244944No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCTCCTACTTGGATAACTGT1210970.10073902766960358Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGGGTACAGTGAAACTGCGAATGGCTC1202940.10007102235800468No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)