FastQCFastQC Report
Sat 4 Jun 2022
EGAF00004838868

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838868
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113699361
Sequences flagged as poor quality0
Sequence length91
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCAT37472563.295758188122095Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGCCGCCTGGATACCGCAG12214911.074316503854406Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATT10713130.9422330878359114Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCATTCGAA10144680.8922372043937872No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAGCGTTTACTTTGAAAAA10001760.8796672129054446Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCCCCCGTGGCGGCGACGA9081880.7987626245322522Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCCCGGCCGGGGGGCGGG8677150.7631661184094077Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGCGACGACCCATTCGA7455030.6556791466928297Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC6817760.5996304587850763Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTCGCCGGCCGAGGTGGGA6469390.5689908846541363Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCCCGGCCGGGGGGCGGGC6129460.5390936190045958Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCATTCGAACG5569740.489865549904014No Hit
CAGTGGTATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCATTCG5347450.4703148683482926Clontech SMARTer II A Oligonucleotide (100% over 22bp)
GCAGTGGTATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCATTC4723510.41543857049469257Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT4547070.39992045337880133Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGCCGATCGCACGCCCCC4499850.39576739573760666Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGTGGCGGCGACGACCCATTC3972280.3493669590632088Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC3787800.33314171396266684Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGGCGGGCGCCGGCGGC3739530.32889630751750665Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGGAGCCGCCTGGATACCGCAGCTAGG3619430.31833336336868245No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTACAGTGAAACTGCGAAT3565250.3135681650840588Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGGCGACGACCCATTCGAA3556950.31283816977652146Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGCGGGCGCCGGCGGCT3510970.30879417167524803Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCATTCGAACGTCT3281970.2886533372865657No Hit
AGTGGTATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCATTCGA3013030.2649997302975168Clontech SMARTer II A Oligonucleotide (100% over 21bp)
GTGGTATCAACGCAGAGTACATGGGGGCCCCGGCCGGGGGGCGGGCGCCG2957220.26009117148864186No Hit
GTGGTATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATTCGAAC2901550.255194925853629No Hit
GTGGTATCAACGCAGAGTACATGGGGCCCCCCGTGGCGGCGACGACCCAT2840540.24982902058701983No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGAGTGTCCCGCGGGGCCCG2775820.24413681621306563Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGGCGCCGGCGGCTTTG2746240.2415352184784926Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGGGGCGGGCGCCGG2636900.23191862969221083Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCTGAGTGTCCCGCGGGGC2567110.2257805125219657Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCAGTGGTATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCATT2515410.2212334333171846Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCCCCTCTCCGGCCCCGGC2480390.21815338082682803Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAGGGACTTAATCAACGCA2459590.21632399499589097Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGCGGCGCCTCGCCTCGGC2453830.2158173958427084Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGCTTTGGTGACTCTAG2444330.21498185904492462Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGGAAGCGTTTACTTTGAAAAAATTAG2402420.21129582249807013No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGCCGGCGGCTTTGGTGA2309750.20314538091379422Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCTTTGGTGACTCTAGATA2169160.19078031581901328Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGGAGCCGCCTGGATACCGCAGCTAGGAA2142670.18845048742182466No Hit
GTGGTATCAACGCAGAGTACATGGGGGCGGCGACGACCCATTCGAACGTC2102980.18495970263192596No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGGGGGGGATGCGTGCATT2091700.18396761262361008Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCTCCTACTTGGATAACTGTG2085810.18344957980898416Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGGCCCCGGCCGGGGGGCGGGCGCCGG2068720.1819464930853921No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATCTCAACCTATTCTCAAA2057020.18091746355549Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATTCCTCGTTCATGGGGAA2044420.1798092779079031Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGACCCCCGCCACGCAGTTTTA2012140.17697021182027575Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGGGGCGGGCGCCGGC1908010.167811849004147Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGGCTCGCCGGCCGAGGTGGGATCCCG1867840.16427884761814976No Hit
GGTATCAACGCAGAGTACATGGGGGCCCCGGCCGGGGGGCGGGCGCCGGC1775460.15615391189401673No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGTGTCCCGCGGGGCCCGA1743070.15330517116978346Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA1736880.15276075298259592Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGGAGCCGCCTGGATACCGCAGCTA1719890.15126646138319105Clontech SMARTer II A Oligonucleotide (100% over 22bp)
GGTATCAACGCAGAGTACATGGGGCCCCCCGTGGCGGCGACGACCCATTC1715670.15089530714249133No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCCGCCTGGATACCGCAGC1635640.14385656925547718Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATTCGAACGT1630250.1433825120617872No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGAGCCGCCTGGATACCGCAGCT1566300.13775803014407442Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCGTTTACTTTGAAAAAA1552840.13657420642847765Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATTCGA1508860.13270611081094819Clontech SMARTer II A Oligonucleotide (100% over 22bp)
GTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCCGGTCG1500670.1319857901400167No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGCCGGGGGGCGGGCGCCG1473470.12959351636109898Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCGCGCGGCGCCTCGCCTCGG1473260.12957504660030586Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCGCCGGCGGCTTTGGTG1468960.12919685626025637Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCGAAGCGTTTACTTTGAAAA1466780.12900512255297547Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGAACTGAGGCCATGATTA1464890.12883889470583743Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGGCCCCCCGTGGCGGCGACGACCC1441880.12681513663036328Clontech SMARTer II A Oligonucleotide (100% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTGGTGACTCTAGATAA1432090.12595409397243668Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGGGCCCCGGCCGGGGGGCGGGCGC1428340.12562427681541674Clontech SMARTer II A Oligonucleotide (100% over 22bp)
GGTATCAACGCAGAGTACATGGGGAAGCGTTTACTTTGAAAAAATTAGAG1380260.12139558110621221No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGGGCGGGCGCCGGCGG1355030.11917657127378228Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATTCG1339180.11778254409011146Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGGGCTCTCGCTTCTGGC1321350.11621437344753414Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGGCTTTGGTGACTCTAGA1317220.11585113481860289Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGGCCCCCCGTGGCGGCGACGACC1302900.11459167303499622Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GTGGTATCAACGCAGAGTACATGGGCGGGCCGATCGCACGCCCCCCGTGG1291630.11360046253909907No Hit
CAGTGGTATCAACGCAGAGTACATGGGGAAGCGTTTACTTTGAAAAAATT1270570.11174820938527526Clontech SMARTer II A Oligonucleotide (100% over 22bp)
ATCAACGCAGAGTACATGGGGAGCCGCCTGGATACCGCAGCTAGGAATAA1261860.11098215406857037No Hit
ATCAACGCAGAGTACATGGGGGCCCCGGCCGGGGGGCGGGCGCCGGCGGC1255930.11046060320426955No Hit
GTGGTATCAACGCAGAGTACATGGGGGGGGGCGGGCGCCGGCGGCTTTGG1251260.11004987090472744No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGGCCCCGGCCGGGGGGCGGGCG1231420.10830491826598744Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GGTATCAACGCAGAGTACATGGGGCCCCGGCCGGGGGGCGGGCGCCGGCG1229280.10811670260838142No Hit
GGTATCAACGCAGAGTACATGGGGGCGGCGACGACCCATTCGAACGTCTG1200010.1055423697587887No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1184970.10421958308103421No Hit
GTGGTATCAACGCAGAGTACATGGGGGGGGCGGGCGCCGGCGGCTTTGGT1157610.10181323710341697No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT1138780.10015711521896768Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)