FastQCFastQC Report
Thu 16 Jun 2022
EGAF00004838876

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838876
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences181250750
Sequences flagged as poor quality0
Sequence length91
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCAT82111574.530274771276808Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATT77639464.283538688805426Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGCGACGACCCATTCGA48021202.649434554063914Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCATTC21445041.1831697248149318Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATTCG20263711.1179931669248264Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GTGGTATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCATTCGAA17918740.9886160471060119No Hit
GTGGTATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATTCGAAC17135100.9453809156651765No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGGCGACGACCCATTCGAA16264990.89737504534464Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGGGCGGCGACGACCCATTCGAAC12609480.6956925695479881Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC11605610.6403068676957199Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGGGCGGCGACGACCCATTCGAACGTC10881930.600379860497129No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGCCGATCGCACGCCCCC10281990.5672798595316157Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCAGTGGTATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCATT9968070.5499602070612122Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CAGTGGTATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCATTCG9453900.521592324445554Clontech SMARTer II A Oligonucleotide (100% over 22bp)
AGCAGTGGTATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATTC9406240.5189628180848906Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCCCCCGTGGCGGCGACGA9256810.5107184384064618Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATTCGA8985180.4957320176606166Clontech SMARTer II A Oligonucleotide (100% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAGCGTTTACTTTGAAAAA8676760.4787158122104322Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGTGGCGGCGACGACCCATTC8383380.46252939643008373Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGTGGTATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCATTCGA6316430.3484912476224236Clontech SMARTer II A Oligonucleotide (100% over 21bp)
AGTGGTATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATTCGAA6002400.33116552621161566Clontech SMARTer II A Oligonucleotide (100% over 21bp)
AGCAGTGGTATCAACGCAGAGTACATGGGGGCGGCGACGACCCATTCGAA5813290.32073191421276875Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTCGCCGGCCGAGGTGGGA5753930.3174568932818209Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGGGCGGCGACGACCCATTCGAACG5648680.3116500207585348Clontech SMARTer II A Oligonucleotide (100% over 22bp)
GGTATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCATTCGAACG5538270.3055584597580976No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCCCGGCCGGGGGGCGGG5349990.29517064067321097Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATTCGAACGT5318720.2934454064328009No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGCGGCGACGACCCATTCGAACG4249040.23442882305314597Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGCCGCCTGGATACCGCAG4196820.23154773152662816Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGTGGTATCAACGCAGAGTACATGGGGGCGGCGACGACCCATTCGAACGT3763080.20761734779028504Clontech SMARTer II A Oligonucleotide (100% over 21bp)
GTGGTATCAACGCAGAGTACATGGGGCGGCGACGACCCATTCGAACGTCT3592300.19819504195155052No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGCCTGGATACCGCAGCT3563100.1965840141351139Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGGGCGGCGACGACCCATTCGAACGTCTG3311080.182679519946814No Hit
ATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCATTCGAACGTCT3289160.18147014564077665No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCCCGGCCGGGGGGCGGGC3275400.18071097636837366Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTACAGTGAAACTGCGAAT3110970.17163901390752867Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATTCGAACGTCTG3076110.16971571152119372No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCTTTGGTGACTCTAGATA2739140.1511243401751441Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACCCA2723280.15024930931320285Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA2707060.1493544164644836Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGCGGGCCGATCGCACGCCCCCCG2664390.14700021930943732Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG2589670.14287775360929542Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGGCCCCCCGTGGCGGCGACGACC2512960.1386454952600196Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC2484280.1370631569800401Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGCGGCGCCTCGCCTCGGC2473930.136492124860173Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGTGTCCCGCGGGGCCCGA2383040.13147752492058654Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGGCGGGCGCCGGCGGC2262250.12481327663471738Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGCGGGCCGATCGCACGCCCCCCGTGG2242080.12370045365329523No Hit
GTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACCCACTC2227420.12289162941394725No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCATGACCCGCCGGGCAGCT2223480.1226742510030993Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATCAACTTTCGATGGTAGTC2207810.12180970285640197Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGGTGGCGGCGACGACCCATTCGA2175980.12005357219211507Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACATGGGGAAGCGTTTACTTTGAAAAAAT2170910.11977384921165843Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GTGGTATCAACGCAGAGTACATGGGGCCCCCCGTGGCGGCGACGACCCAT2151300.11869192265411316No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGAGTGTCCCGCGGGGCCCG2015290.11118795370501915Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCAACGCAGAGTACATGGGGGCGGCGACGACCCATTCGAACGTCTGCCC1968210.10859044721194258No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGGCGGCGACGACCCATTCGAAC1968010.1085794127748437Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GTGGTATCAACGCAGAGTACATGGGGTGGCGGCGACGACCCATTCGAACG1963340.10832175866858482No Hit
GTGGTATCAACGCAGAGTACATGGGGAAGCGTTTACTTTGAAAAAATTAG1953750.10779265740969349No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGCGGGCGCCGGCGGCT1925680.10624397416286553Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGGCGGCGACGACCCATTCGAACGT1881950.10383129449119521Clontech SMARTer II A Oligonucleotide (100% over 22bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)