FastQCFastQC Report
Thu 16 Jun 2022
EGAF00004838878

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838878
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115639420
Sequences flagged as poor quality0
Sequence length91
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATT30980612.6790699918764727Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCAT15081001.3041400588138543Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGCGACGACCCATTCGA12425381.0744934556053636Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAGTTGCTGCAGTTAAAA6952270.6012024273383593Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGCCGCCTGGATACCGCAG6461780.5587869603635162Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATTCG6283010.5433276991531089Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGTGGCGGCGACGACCCATTC6257210.5410966260467235Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTCGCCGGCCGAGGTGGGA4610100.39866163285841455Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCAGTGGTATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATTC3762920.3254011478092851Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GTGGTATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATTCGAAC3749210.3242155659376361No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGATCAAAACCAACCCGGTC3661080.31659446233818883Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAGCGTTTACTTTGAAAAA3478410.3007979458907698Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGCCGATCGCACGCCCCC3182950.27524783503756767Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCATTC3075770.2659793693188707Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCCCCCGTGGCGGCGACGA2884350.24942619048072015Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATTCGA2742330.237144911311385Clontech SMARTer II A Oligonucleotide (100% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA2690920.2326991954819559Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGGGCGGCGACGACCCATTCGAAC2536760.21936810129279447Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGC2525850.21842465138617956No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTGGAGGGCAAGTCTGGTG2518190.21776224751040776Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCCCGGCCGGGGGGCGGG2352830.20346262546111007Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAACGAGGATCCATTGGAGGG2336730.20207036666216416Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAACCTCGGGCCGATCGCAC2240660.19376264599044166Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGGCGACGACCCATTCGAA2225560.19245686289329364Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAACGAGGATCCATTGGAGG2144120.18541428173887417Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAATCCTTTAACGAGGATC2042160.17659721918356214Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGATAACCTCGGGCCGATCG1986300.17176668648113247Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAATACAGGACTCTTTCGAGG1970820.17042804261730127Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGACCCTTCTCCACTTCGGCCT1923680.1663515780345491Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGTGGTATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATTCGAA1908110.1650051513575561Clontech SMARTer II A Oligonucleotide (100% over 21bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGACCCACTCCCGACCCGGGGA1892170.16362672867089786Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCAGTGGTATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCATT1846060.15963933406099753Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GTGGTATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCATTCGAA1827730.15805423444704236No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAACGAGGATCCATTGGAGGG1801200.15576003407834457Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAAAGTTGCTGCAGTTAAAAA1793040.15505439235167384Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT1789220.15472405517080595No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC1759610.15216350964057068Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTC1713520.14817784454470628No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCTGAGTGTCCCGCGGGGC1680220.1452982036748368Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAACTTTCGATGGTAGTCGCC1677760.14508547344841405Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTA1619740.14006815323010094No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCCCGGCCGGGGGGCGGGC1579040.13654859216692716Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGGGCGGCGACGACCCATTCGAACGTC1541280.13328326966703916No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGAGTGTCCCGCGGGGCCCG1530540.13235452062972988Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCA1518710.13133151307746094No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACCCCCGCCACGCAGTTTTA1515220.13102971287818635Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCAGTGGTATCAACGCAGAGTACATGGGGGCGGCGACGACCCATTCGAA1511340.13069418715521058Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCATTGGA1460430.12629170917668042No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGACCGCGGTTCTATTTTG1442490.1247403350864264Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCCGCGGTAATTCCAGCTC1427790.12346914226999756Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCTCCTACTTGGATAACTGTG1426040.12331780979185127Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAACCTCGGGCCGATCGCACG1422810.12303849327504411Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1421660.12293904621797652Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC1395190.1206500343913866Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATTCGAACGTCTGCCCTATC1392990.12045978784743126Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAGCTCGTAGTTGGAT1390440.12023927480784666Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT1380940.11941775564076679No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAGAAATCACATCGCGTCAA1377570.11912633252570792Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGGAGCCGCCTGGATACCGCAGCT1359130.117531720584555Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAAATCCTTTAACGAGGATCC1357560.1173959537327323Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCATTCG1341910.11604260899959547Clontech SMARTer II A Oligonucleotide (100% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAGTCTGGTGCCAGCAGCC1309140.1132088002516789Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGGTGGCGGCGACGACCCATTCGA1253730.10841718161505826Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCGAGGCCCTGTAATTGGAAT1249260.10803063522802173Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAAAGTTGCTGCAGTTAAAAAG1247500.10787843799285744Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGA1235850.10687099606691212No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCCAAGGAAGGCAGCAGGC1235550.10684505335637276Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATCAACTTTCGATGGTAGTC1234820.10678192609406031Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAG1215450.10510689175023534No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCCCCTCTCCGGCCCCGGC1213560.10494345267383735Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAACAATACAGGACTCTTTCG1212850.10488205492556084Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA1207900.10445400020166133Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAACCTCGGGCCGATCGCACG1205650.1042594298726161Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAATCGGTCGTCCGACCTG1195210.10335662354584622Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT1193040.1031689712729448Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAATGTTTTCATTAATCA1179610.10200760259779927Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTAT1177950.10186405293281478No Hit
GGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATA1160900.10038964221716089No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGAATAATGGAATAGGACC1159800.10029451894518324Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)