FastQCFastQC Report
Thu 16 Jun 2022
EGAF00004838880

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838880
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109173531
Sequences flagged as poor quality0
Sequence length91
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATT29234242.6777772718553914Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCAT14236211.3039983107260678Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGCGACGACCCATTCGA11710051.072608890885832Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAGTTGCTGCAGTTAAAA6567060.6015249245716894Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGCCGCCTGGATACCGCAG6206030.5684555535718635Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATTCG5934530.5435868882907158Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGTGGCGGCGACGACCCATTC5867790.5374736848989523Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTCGCCGGCCGAGGTGGGA4346050.39808641895076197Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCAGTGGTATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATTC3556680.3257822631018502Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GTGGTATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATTCGAAC3528730.32322211873865286No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAGCGTTTACTTTGAAAAA3352700.30709824710167155Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGATCAAAACCAACCCGGTC3307500.30295804941950627Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGCCGATCGCACGCCCCC3004030.2751610186538713Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCATTC2882680.26404568704478376Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCCCCCGTGGCGGCGACGA2737390.2507375162208503Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATTCGA2585860.23685777828327273Clontech SMARTer II A Oligonucleotide (100% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA2443970.22386103825844014Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGC2442380.22371539856121353No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTGGAGGGCAAGTCTGGTG2422360.2218816207382722Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGGGCGGCGACGACCCATTCGAAC2389460.2188680697705014Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAACGAGGATCCATTGGAGGG2263360.2073176510156111Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCCCGGCCGGGGGGCGGG2129070.19501705042406295Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAACCTCGGGCCGATCGCAC2110800.19334356786536472Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGGCGACGACCCATTCGAA2092500.1916673373878509Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAACGAGGATCCATTGGAGG2077580.19030070576356095Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAATCCTTTAACGAGGATC1975640.1809632776281643Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAATACAGGACTCTTTCGAGG1920040.17587046808992557Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGATAACCTCGGGCCGATCG1865140.17084177665738412Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGACCCACTCCCGACCCGGGGA1808510.16565462190647656Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGACCCTTCTCCACTTCGGCCT1807750.16558500796314815Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGTGGTATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATTCGAA1795370.16445103346524534Clontech SMARTer II A Oligonucleotide (100% over 21bp)
AGCAGTGGTATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCATT1748990.16020275097633327Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAACGAGGATCCATTGGAGGG1740610.15943516565384333Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT1737350.15913655847588187No Hit
GTGGTATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCATTCGAA1718600.15741910921613408No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAAAGTTGCTGCAGTTAAAAA1698990.15562288628367255Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC1679180.15380834389244039Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTC1667420.15273115971672657No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCTGAGTGTCCCGCGGGGC1599900.14654651043575756Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAACTTTCGATGGTAGTCGCC1570730.1438746173740593Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTA1559040.14280384500891521No Hit
CGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCA1463040.13401050479900664No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGAGTGTCCCGCGGGGCCCG1461750.133892344289936Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGACCCCCGCCACGCAGTTTTA1448930.13271806698273778Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGGGCGGCGACGACCCATTCGAACGTC1443830.1322509207840864No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCCCGGCCGGGGGGCGGGC1440580.13195322957906344Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCAGTGGTATCAACGCAGAGTACATGGGGGCGGCGACGACCCATTCGAA1423180.13035943666601751Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCATTGGA1418490.12992984535784594No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGACCGCGGTTCTATTTTG1387490.1270903292483963Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCCGCGGTAATTCCAGCTC1383500.12672485604592199Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC1348240.12349513546465764Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1343450.12305638442710073Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAACCTCGGGCCGATCGCACG1340330.1227706008702787Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT1329830.12180882928481997No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTCGAACGTCTGCCCTATC1321290.12102658839531352Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGGAGCCGCCTGGATACCGCAGCT1316990.1206327200317447Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAAATCCTTTAACGAGGATCC1312460.12021778429058963Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAGCTCGTAGTTGGAT1312180.12019213704831073Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCTCCTACTTGGATAACTGTG1295380.11865330251157674Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCATTCG1261990.1155948688698179Clontech SMARTer II A Oligonucleotide (100% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAGTCTGGTGCCAGCAGCC1256630.11510390737476468Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCAGAAATCACATCGCGTCAA1255850.11503246148555916Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCGAGGCCCTGTAATTGGAAT1207950.1106449511099902Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGA1198170.10974912957610578No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCCAAGGAAGGCAGCAGGC1181450.10821762282288003Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAAAGTTGCTGCAGTTAAAAAG1177970.10789886424027084Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAG1176610.10777429192063047No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGTGGCGGCGACGACCCATTCGA1173480.1074875923908699Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAACAATACAGGACTCTTTCG1171660.10732088531605706Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATCAACTTTCGATGGTAGTC1150390.10537261087579919Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAACCTCGGGCCGATCGCACG1145620.10493569178411936Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAATGTTTTCATTAATCA1141300.10453999147467348Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT1133850.10385759163546703Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTAT1131900.10367897691245324No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAATCGGTCGTCCGACCTG1125560.10309825006942389Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATA1113300.10197526724678348No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGTCTGGTGCCAGCAGCCG1110380.10170780314873208Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGAATAATGGAATAGGACC1109580.10163452531364951Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA1109410.10161895377369447Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)