FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842256

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842256
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37261048
Sequences flagged as poor quality0
Sequence length35-86
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT775650.20816644770699955No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG705960.18946327006153987No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC499750.13412129470969256No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA399230.10714406100440332No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGCAC223350.028.83174180
CGCGTTC24550.026.0062488
CCGCGTT25750.024.794247
ACCGCGT26200.024.3684166
GACCGCG26600.023.7038145
ATAGCGC259600.021.09796178-79
GGAGTCT359350.019.2344153
TGGAGTC375600.018.4762572
GAGTCTT375300.018.4063744
CTCGCTA91600.017.1965541
AGTCTTG428300.016.7954625
CGATCGG305200.016.72117470-71
CGCTATG104750.016.6286491
CGGCATA317550.016.17193874-75
ATCGGCA328250.015.71253672-73
TCGCTAT103250.015.4396542
CCCGATC344650.014.93417968-69
CACCCGA344000.014.90578966-67
CCTACGT229700.014.81550224-25
GTCTTGG503650.014.7236526
Produced by FastQC (version 0.11.5)