FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842257

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842257
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24326233
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCAAT28650.034.47681833
TCTCGTA29400.033.3728341
CTCGTAT30150.032.39672542
TATCTCG31400.031.3172339
CGTATGC31550.030.88942544
GTCACGC32000.030.45504429
ACGCCAA32550.030.34595732
GATCGGA33750.029.8205661
TCGTATG33500.029.3540643
ATCTCGT34750.028.10823840
CGTCTGA35900.027.82074216
AGTCACG35000.027.78175528
ATCGGAA37100.027.3384862
ACACGTC37050.027.19678513
TCGGAAG37450.027.0217933
ACGTCTG41850.024.12901915
CGGAAGA42350.023.843214
CACGTCT44200.023.04618314
GCACACG53800.019.46543311
CACACGT58500.017.9015812
Produced by FastQC (version 0.11.5)