FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842259

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842259
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25948926
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA23800.030.3489441
CTCGTAT25450.028.52544642
ATCGGAA27250.026.9661462
TCTCGTA27150.026.3341841
TCGTATG29350.024.51014743
CGTATGC32500.022.20223844
CGTCCAT34000.021.15802635
TCGGAAG35500.020.883843
ATCTCGT36700.019.72132740
CGGAAGA38850.019.3660644
CCGTCCA41450.017.83286334
ACCCGTC40850.017.82552132
CGTCTGA42550.017.42371716
CATCTCG47950.015.41547639
TCACCCG49450.014.99235630
ACACGTC52000.014.51216513
CACCCGT50650.014.41998931
ACGTCTG60450.012.55593815
CCCGTCC64300.011.63253233
GTCCATC64000.011.58393836
Produced by FastQC (version 0.11.5)