FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842261

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842261
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32147358
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG6850.020.23239132
CGTCCGC7500.018.185633
GATCGGA10250.014.621581
CTCGTAT12200.011.72151842
GTCCGCA12050.011.68365434
TCTCGTA13450.010.63202541
CGTATGC14050.010.17794144
TCCGCAT13850.010.16550135
ATCGGAA15650.09.4179642
CCGCATC15500.08.94199636
TCGGAAG18050.08.6532193
CGCATCT15550.08.63028337
ACACGTC18150.08.24176213
CGTCTGA18550.08.06429316
GTCACGT19600.07.74466129
ACGTCTG22450.07.643275715
ATCTCGT20300.07.477882440
CGGAAGA23150.07.4120244
AGTCACG19350.07.389954628
TCGTATG19000.07.29466143
Produced by FastQC (version 0.11.5)