FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842266

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842266
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29590525
Sequences flagged as poor quality0
Sequence length86
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCTTGT1458150.4927759815008352No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA1389270.4694982600004562No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1307440.44184413760823776No Hit
GGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCT1259800.4257443894625053No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAG1114030.3764819988830884No Hit
GCTTGATCTTGATTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCA1071380.3620686013512771No Hit
CCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGGCA993790.3358473700618695No Hit
GTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGT959780.32435382609804997No Hit
GTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGTC940840.3179531285774754No Hit
GTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGG904000.30550319739173265No Hit
CTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTT886580.29961617781367517No Hit
GTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGA881680.29796024234108726No Hit
GGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTG849960.28724059475119146No Hit
GGGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCC673380.22756608745535944No Hit
GCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCGGCTCTT631450.21339601105421413No Hit
GCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATG617160.208566762502524No Hit
GGGTAAACGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTC596020.20142258374935898No Hit
GTCGCTTTTTGATCCTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGA582340.19679948226670532No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG574000.1939810125031577No Hit
CGACTGTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGA558030.18858401464658028No Hit
CCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAAC527240.17817865685046144No Hit
GTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGA517660.1749411340285446No Hit
GTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACAT487500.16474868222175848No Hit
GGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCAG475020.16053111595688146No Hit
GTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAG437980.14801359556817598No Hit
GCAGATCTTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCG428540.1448233851883331No Hit
GTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATGTGTTG425460.1437825114627064No Hit
TGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGC416610.1407916892316037No Hit
GGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACGATCTCAACCTATT411120.1389363656102756No Hit
GCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTG395060.13350895261236495No Hit
GGGGAATCCGACTGTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCG391700.13237345400259035No Hit
GGTAAACGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCG378980.128074780694158No Hit
GCCACTTTTGGTAAGCAGAACTGGCGCTGCGGGATGAACCGAACGCCGGG373650.12627352843520012No Hit
GCCTAGTGGGCCACTTTTGGTAAGCAGAACTGGCGCTGCGGGATGAACCG350840.11856497983729589No Hit
CAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAACG349380.11807157865566766No Hit
GTGGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCG345200.11665896431374569No Hit
GGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGTCCGTAGCGGTCCTGA330810.1117959211605742No Hit
GGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCGGC329030.11119437725420553No Hit
GCCAGGTGGGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCA328960.11117072103316857No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA325610.11003860188354211No Hit
GCTTTTTGATCCTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATT319550.1079906490337701No Hit
GCCGAAACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCG315860.10674362823910695No Hit
GAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTTGGGT315680.10668279795644044No Hit
GCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATGGATGAACGAG315150.10650368656858909No Hit
AGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAA299410.10118441629541888No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG625300.040.2992551
CCGCGTT17200.029.2919397
CGCGTTC18100.028.4981848
ACCGCGT18150.026.6572156
GGGAGTT414450.025.5446821
AGGGGGG180650.025.1607271
CGACGCG4700.024.687831
GCGAGTG109650.024.5917480
GCCTAGT138650.023.9230981
CCTAGTG143250.022.7214762
GAGTTTG470900.022.3661333
CTAGTGG143600.022.2761123
GTCGCGA3300.021.8243521
GGAGTTT485800.021.7790582
GCGTATA29500.021.56319480
AGTTTGA493300.021.528814
TATGCCG25750.021.51730248-49
GCCCTAT99150.021.2263741
CCCTATC96800.021.0669462
CGCCTCG26450.020.5597153
Produced by FastQC (version 0.11.5)