FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842267

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842267
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29289084
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA29350.034.9634481
ATCGGAA32750.031.1703592
CTCGTAT34300.029.63152742
TCGTATG36400.028.1033343
TCTCGTA36550.027.68704641
CGTATGC41350.025.00509844
TCGGAAG42500.024.6909143
ATCTCGT45400.022.19302740
CGGAAGA46900.021.8115394
CGTCTGA51100.020.27735916
CATCTCG53750.019.48208439
ACACGTC59250.017.63796813
CTTAGGC68850.015.24125233
ACGTCTG69900.015.01297315
ACTTAGG78450.013.65658832
CACTTAG86150.012.30829731
CACGTCT89600.012.0318214
TTAGGCA89300.011.99732734
TAGGCAT91750.011.55707535
GCACACG99850.011.28145711
Produced by FastQC (version 0.11.5)