FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842269

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842269
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28067422
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA29500.034.0389671
ATCGGAA32750.030.901632
CTCGTAT34000.028.98713742
TCTCGTA35150.027.9761841
TCGTATG36600.026.86783643
CGTATGC37800.026.5386844
TCGGAAG38900.026.1276843
ATCTCGT42850.023.15431640
CGGAAGA43900.022.9513364
CGTCTGA47100.021.48557716
CATCTCG50250.019.61318839
ACACGTC53700.018.72342513
ACGTCTG62950.016.60064715
GATCATC72450.013.96772436
CACGTCT78300.013.76821614
CCAGATC84300.012.36963833
ACCAGAT83400.012.26572232
GCACACG92100.011.60967311
TCTGAAC93050.011.20645318
GTCTGAA98750.010.91613917
Produced by FastQC (version 0.11.5)