FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842271

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842271
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23860579
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGA20100.038.4164934
CCGTACG21650.035.8693433
GATCGGA23900.033.6300621
TACGATC24800.031.49076736
ATCGGAA26900.030.1801012
CTCGTAT26800.029.3869742
GTACGAT26700.029.08506635
TCTCGTA27700.028.67041841
TCGTATG28600.027.6143643
ACCGTAC28700.027.51814532
TCGGAAG33350.024.2753183
CGTATGC33700.023.9575744
ATCTCGT37050.021.79136740
CGGAAGA37700.021.474054
CACCGTA38600.021.0303131
CGTCTGA39200.020.7085916
GTCACCG45100.018.29200629
ACACGTC49800.017.00868613
ACGTCTG58000.014.71725115
ACGATCT62450.013.56234837
Produced by FastQC (version 0.11.5)