FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842276

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842276
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44102490
Sequences flagged as poor quality0
Sequence length35-43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGG754130.17099488033442103No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCG723880.16413585718176002No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN459920.10428436126849072No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATTCG124800.035.9155737
CGTTACC45400.027.6565737
TGCGACA112650.025.715214
GCGACAT114900.025.1641645
TCGATCG49000.024.59804716
TTGATCG118300.024.0860523
TGATCGC121400.023.5184324
CGCGTTA34400.023.39176235
CGACATC131400.021.9772876
CTGCGAC135300.021.679843
GCTGCGA134300.021.6646182
GATCGCC133050.021.51632725
TCGGCCG56900.021.50690820
ATCGGCC60600.020.19270319
TTCGATC62200.020.02288215
GGTGTGC333300.019.0303047
TCCAACG46200.018.90620437
CGGCCGA66700.018.75851821
CTTTCGA66500.018.4540913
TTTCGAT67150.018.22110714
Produced by FastQC (version 0.11.5)