FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842279

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842279
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29843300
Sequences flagged as poor quality0
Sequence length98
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG864120.28955242885337745No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA518510.17374419048831732No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG356660.11951091199699765No Hit
GGGCCAGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA352890.11824764687551309No Hit
GGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA319390.10702234672439041No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA312510.10471697164857773No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCATAG537750.054.74983292
TCACCCC651800.046.1533939
GTAGCAA810500.044.5293929
TCCGACC173750.044.309039
TAGTAGC826250.043.091057
AGTAGCA847550.042.5015268
TTCCGAC183250.042.0871738
TCCTGAG827950.041.56223392
TTTCCGA190050.040.8227847
AGTACGA111000.040.3482449
CAGTACG114700.039.4872138
GTAGTAG676350.039.377456
GGTAGTA581250.038.200455
GATTTAA374750.037.679327
GTTTCCG177650.036.6132976
CAATTCA381050.036.54705492
ACCCGAT473200.036.05006884-85
CCGATCG477850.035.7004986-87
CGATCGG462550.035.6647486-87
TTTAATC401250.035.637499
Produced by FastQC (version 0.11.5)