FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842280

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842280
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35650719
Sequences flagged as poor quality0
Sequence length35-43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGG667530.18724166544859866No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCG617550.17322231285153042No Hit
GGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTC383670.10761914787749442No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN363550.10197550293445695No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATTCG108100.034.71702637
TGCGACA93150.026.1491624
GCGACAT97400.024.8958665
CGTTACC27450.024.13895437
TTGATCG102850.023.46772623
TCGATCG26350.023.32498716
CGCGTTA18650.023.24566735
CGACATC107250.022.6608756
TGATCGC107700.022.44640424
TAAGTTC122750.022.01113737
GTATCTC86900.021.92193437
CTGCGAC119050.020.8585663
GCTGCGA121550.020.444422
GGTGTGC335650.020.2534587
GCGAGTA58600.020.2232633
GATCGCC121000.020.0860325
AAGTTCG68100.019.50197837
GTGCTCT372100.019.31182710
TGTGCTC401450.018.9540279
CGAGTAT63300.018.73487534
Produced by FastQC (version 0.11.5)