FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842290

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842290
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37606950
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGGCGGGTGTCCCCTT520640.13844249533663325No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGTGC619950.035.8781287
CGACTAA73400.034.5924967
GCGACAT114200.034.1933565
TCGATCG78800.033.88255316
TGCGACA118050.033.2892534
GTGTGCT859100.031.9257978
ACGACTA80400.031.5807166
TCCGATC327200.030.06752217
TTCCGAT336350.030.05977616
GTGCTCT530450.030.01172410
TGTGCTC645600.029.9164899
GCGAGTA85750.029.73074533
TCTTCCG386500.029.70280614
CTTCCGA368300.029.4939115
CTCGCGT21950.029.38805210
CGCGTTA81350.029.30096235
GCACGAC87550.029.11979964
CGGTCGA47150.029.04700569
TCGGCCG93850.028.85422920
AACGGCG93050.028.77117359
Produced by FastQC (version 0.11.5)