FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842292

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842292
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29770618
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTAA66150.035.8805867
GGTGTGC428700.033.4090847
ACGACTA72150.033.0401966
GCACGAC78550.030.21642764
GTGCTCT386550.029.5293610
GTGTGCT572100.029.3824088
GCGAGTA81700.029.2201233
TCTTCCG273750.029.0581614
TCGATCG34800.028.64701516
TGTGCTC453850.028.5328319
TTCCGAT228700.027.97940416
CTTCCGA264500.027.81800315
CGGTCGA35000.027.10568669
CACGACT88400.027.08370665
CGAGTAT90250.026.52834934
TCCGATC227900.026.3823717
TCGAAAG92600.026.15196845
TGCGACA63300.025.2904594
GCGACAT64500.024.922235
CCGATCT234600.024.3496718
Produced by FastQC (version 0.11.5)