FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842297

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842297
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41980337
Sequences flagged as poor quality0
Sequence length35-43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG960410.22877615298800483No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCT781010.1860418605024538No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCG632390.1506395720453602No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN584170.13915324214762737No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAAC523780.12476793599822697No Hit
GGGCCAGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCT514010.12244065596710194No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGCT542650.041.28840337
GGCTCGC76250.031.29147137
GTACCGT22450.030.26098337
CGGATAG68500.029.8406235
TCGGATA67900.029.53211234
TCCGACC118600.029.0059839
CCCCCGC591450.028.17617836
CCGACCT123400.027.8337210
TCCCCCG582150.026.32643935
CGGCTCG67100.026.03411536
GGCAACC593550.025.44126722
GTCCCCC596800.025.32473634
ATCGCGA31200.025.28977219
CAACCTG603850.025.05508424
AACCTGG607350.024.89293525
AAGGCCG312300.024.87843736
ACCTGGT610000.024.7469826
CGACCTG139250.024.67877411
GTGGTCC613700.024.58467131
GGTCCCC614950.024.56144133
Produced by FastQC (version 0.11.5)