FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842299

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842299
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42284779
Sequences flagged as poor quality0
Sequence length35-43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG938430.22193092223563474No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCT758920.1794782940688894No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAAC505460.11953710341018928No Hit
GGGCCAGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCT498170.11781307879130691No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGCT510150.041.57668737
TCCGACC117350.029.2286459
CCCCCGC552600.028.39628836
CCGACCT123650.027.72519110
TCGGATA51300.027.46368634
CGGATAG52150.027.38968735
CGACCTG130400.026.47220611
TCCCCCG548800.026.33760635
GTCCCCC558400.025.53381734
GGCAACC560100.025.39535522
CAACCTG572100.024.86763824
GGTCCCC574350.024.74029233
AACCTGG575100.024.6886625
GTGGTCC574600.024.64033131
ACCTGGT576500.024.62548626
TTCCGAC141700.024.5150498
GCAACCT586400.024.32033323
TGGTCCC596300.023.79087432
TTTCCGA145900.023.6842677
GTCGGTT50000.023.5237143
Produced by FastQC (version 0.11.5)