FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842307

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842307
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21205440
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC214220.10102124737803131TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT31450.034.7775942
TCTCGTA32100.032.52903741
CGCCAAT34550.032.1040533
CGTATGC34400.031.92696444
GATCGGA35300.031.6711251
ATCGGAA36950.030.3224742
CGTCTGA37100.030.14278816
TCGTATG37000.030.10930343
ACGCCAA36900.029.99794632
TATCTCG34800.029.81172839
TCGGAAG39000.029.0119763
GTCACGC38600.028.7371129
ACACGTC39200.028.3477713
ATCTCGT38850.027.10942540
ACGTCTG41500.026.94533715
AGTCACG41650.026.9021928
CGGAAGA43400.026.174334
CACGTCT46050.024.42409114
GCACACG49500.022.63091911
GTATGCC51250.021.69389345
Produced by FastQC (version 0.11.5)