FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842308

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842308
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31522497
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCAAT31100.025.6086433
TATCTCG31700.024.91611939
CTCGTAT35050.023.4127442
CGTATGC35100.023.1897744
TCTCGTA35050.023.16236341
ACGCCAA36700.021.70115932
ACACGTC42750.020.73874513
GTCACGC38500.020.5144429
CGTCTGA43100.020.46798716
TCGTATG40100.020.40935743
ATCTCGT41550.019.85659640
GATCGGA46050.018.9703041
ACGTCTG47550.018.64500815
ATCGGAA49750.018.0444932
AGTCACG46600.017.08960728
TCGGAAG53000.016.9791013
CACGTCT55450.016.26656514
GCACACG60850.015.03973111
CACACGT63300.014.38814112
CGGAAGA63600.014.2164124
Produced by FastQC (version 0.11.5)