FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842309

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842309
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31080044
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT16700.012.77830342
CGTATGC18700.012.23510144
TCTCGTA18550.011.97813241
GATCGGA21350.011.667571
TCGTATG20250.011.19001943
CGTCCAT22200.010.30564635
ATCGGAA24450.09.987382
ACCCGTC24100.09.67568232
ACACGTC25350.09.19902113
CGTCTGA25750.09.14151516
ATCTCGT25350.09.1121840
CACCCGT28550.08.93808831
CCGTCCA27300.08.86388734
TCGGAAG32050.08.3054813
ACGTCTG32200.07.515336515
TCACCCG33850.07.148694530
CATCTCG31800.07.056412739
CGGAAGA40150.06.30114844
CACGTCT40150.06.246446614
GCACACG46950.05.950707411
Produced by FastQC (version 0.11.5)