FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842310

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842310
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31938606
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG23050.029.5856732
GATCGGA25550.027.5273931
CGTCCGC25400.026.84849733
GTCCGCA27500.024.7984434
CTCGTAT27150.024.39037942
TCTCGTA27350.024.13113241
CGTATGC27650.023.7104444
ATCGGAA31200.022.7046992
TCCGCAT30350.022.3973135
ACACGTC32900.021.59793513
TCGGAAG33300.021.4710643
TCGTATG31350.021.19294543
CGTCTGA33850.020.92666616
CGCATCT32600.020.31208237
CCGCATC34300.019.88219536
ATCTCGT34450.019.34938440
AGTCACG35850.019.26769628
GTCACGT37050.018.82176429
CGGAAGA39300.018.6408674
ACGTCTG38450.018.53751815
Produced by FastQC (version 0.11.5)