FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842311

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842311
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21887724
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA7800.016.9393561
CTCGTAT9500.014.12704742
CGTAGAG10200.013.15766333
TCTCGTA10300.013.0298641
CGTATGC10800.012.6295944
TCGTATG10950.012.45712343
CGTCTGA11650.012.27368616
ATCGGAA12700.010.9131282
ACGTAGA12300.010.7310632
ACACGTC14200.09.75986413
CACGTAG13450.09.64986631
TCGGAAG14100.09.5177223
ATCTCGT15150.09.43949640
ACGTCTG16150.09.12623915
TCACGTA14500.08.9509630
GTCACGT16250.08.79924929
CACGTCT19250.08.22794314
AGTCACG18400.07.771075728
CGGAAGA20900.06.8420994
GCACACG23500.06.178351411
Produced by FastQC (version 0.11.5)