FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842312

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842312
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36765080
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCAAT33300.027.08623133
TCTCGTA33700.026.04644441
TATCTCG35150.025.66043339
GATCGGA36850.025.0201931
CGTATGC37350.024.32567444
CTCGTAT36950.023.93409742
CGTCTGA39400.023.22876716
ACGCCAA39250.023.03624732
GTCACGC40250.022.40929229
TCGTATG40750.022.18807643
ATCGGAA44200.021.0068052
ATCTCGT42750.020.99565940
TCGGAAG45400.020.4030973
ACACGTC46600.019.40503713
ACGTCTG48600.019.10427915
AGTCACG49450.018.32909828
CGGAAGA53950.017.0880454
CACGTCT56950.016.49653614
GCACACG63900.014.73596211
CACGCCA66250.014.0463731
Produced by FastQC (version 0.11.5)