FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842316

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842316
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31927329
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA20850.027.0405311
CGTATGC18800.021.41509244
TCGGAAG26650.021.3807263
CTCGTAT19400.021.32105442
TCTCGTA19450.021.26627741
ATCGGAA27750.020.611772
CGTCTGA27150.020.49905416
ACACGTC27200.020.38081713
CGGAAGA31400.018.0763174
ACGTCTG34600.016.59399615
TCGTATG25750.016.14830443
CACGTCT36200.015.86055914
CATCTCG28100.015.66044439
ATCTCGT27100.015.1818840
GCACACG41700.014.0854911
CACACGT50200.011.56906612
CCAGATC74200.07.91702333
AGATCGG10600.07.27182671
AGTCACC83350.07.2314528
AGAGCAC88250.07.0551228
Produced by FastQC (version 0.11.5)