FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842318

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842318
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35254850
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC5900.011.9318915
CGACGCT6300.010.8246686
CACGACG6450.010.5733674
GATCGGA15350.010.196731
CTCGTAT14350.09.81193842
TCTCGTA14650.09.6108641
CGTAGAG16150.08.85381833
CGTATGC16850.08.48673744
ATCGGAA20050.08.0097662
ACGTAGA20250.07.712995532
TCCGATC10200.07.54880115
CACGTAG22050.07.48242631
TCGTATG19100.07.25662243
CGTCTGA22350.06.988611716
TCGGAAG24150.06.83211953
GTCACGT24550.06.810074329
TCACGTA23750.06.761592430
ACACGTC23900.06.627423313
ATCTCGT23200.06.543015540
AGTCACG26350.06.3448728
Produced by FastQC (version 0.11.5)