FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842321

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842321
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23168482
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC781030.33710883604717823TruSeq Adapter, Index 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA101400.040.784721
ATCGGAA109700.037.9017682
TCGGAAG111400.037.0913663
CGTCTGA110900.036.406516
CTCGTAT107650.036.08503742
CGTATGC108450.035.88099744
TCTCGTA104250.035.77316341
ACACGTC113650.035.5923213
TCACGAT106000.034.82160635
ACGTCTG116550.034.77671415
TCGTATG112600.034.7184443
CGGAAGA120000.034.4518974
ATCTCGT108300.034.4146140
CACGTCT119000.034.04182414
CATCACG116300.033.26555633
ACGATCT112000.032.97631537
GCACACG123850.032.87911
CACGATC113300.032.57802236
CACACGT129600.031.50704612
GTATGCC127500.030.7492945
Produced by FastQC (version 0.11.5)