FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842322

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842322
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21463787
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC1803500.8402524680290575TruSeq Adapter, Index 9 (100% over 51bp)
GATCGGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC650040.3028542912767444TruSeq Adapter, Index 9 (100% over 46bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGG91650.042.730541
ATCGGGA91150.041.9373932
TCGGGAA93150.040.7585453
GATCGGA264750.040.1011921
CGGGAAG98300.038.0511554
ATCGGAA278300.038.0293432
TCGGAAG266300.037.9315873
CGGAAGA271050.037.0345084
GTATGCC268250.035.39172445
CGTATGC346300.027.39556144
ACACGTC359100.027.2890613
CGTCTGA359200.027.19427916
GCACACG362750.027.0640911
ACGTCTG363350.026.90844215
CACGTCT364500.026.8729114
CACACGT370150.026.56557312
GTCACGA356700.026.51502229
CTCGTAT357650.026.46327242
TCTCGTA338900.026.46066541
AGTCACG359050.026.44797328
Produced by FastQC (version 0.11.5)