FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842325

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842325
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29898530
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGA14950.012.5076410
CTTATAC394850.011.95078444
TCTCTTA981850.011.93769144
TTATACA307550.011.56632944
GTCTCTT1334250.011.04356244
CTCTTAT704300.010.42629244
TCTCCGA45750.010.24203243
TCGTTCG9100.010.15451723
GTATCGA10150.09.96986811
TCTTATA530100.09.91839444
TATACCG13600.09.8670786
CCGTGCG28750.09.6411839
CGGTCGT12600.09.6026210
CGGTATG13050.09.44006843
TATACAC237250.09.39300344
TCGTTGT19700.09.26851634
TATACTG66300.09.2573845
CGTATCG16250.09.20562310
CATCGTT21250.09.11013932
GTATAGA57700.08.9657991
Produced by FastQC (version 0.11.5)