FastQCFastQC Report
Sat 4 Jun 2022
EGAF00004842329

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842329
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58503430
Sequences flagged as poor quality0
Sequence length35-86
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC2717920.46457447024900933No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG1934160.3306062567613557No Hit
ACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCT1228340.20996033907755493No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG1204920.20595715499074158No Hit
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT999060.17076947454192No Hit
ACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCT805070.13761073496032628No Hit
GAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTAT671670.11480865309948494No Hit
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC646930.1105798412161475No Hit
TGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGA610960.10443148376086667No Hit
TAGGCAAAATACCAGCTGATGAAGGCATCTGATGCCTTCATCTGTTCAGT590350.10090861339241135No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)