FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842351

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50564200
Sequences flagged as poor quality0
Sequence length35-42
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG100600.011.2831835
TACACTG114250.09.578645
ATACTGT128500.09.5089416
TCTATAC61050.08.9910743
CTAGGAC85250.08.8481143
TAGACAG131600.08.8136745
TAGACTG86800.08.6704335
TACACCG20700.08.6401775
GTATAGA70650.08.599191
GTATTAG74150.08.5516011
GTGTAGG91250.08.4825061
CTAGTAC38100.08.4582773
CTAGACT88400.08.3729154
TCTGTCG49850.08.2424348
GTGTAAG93650.08.1894681
CTATACT78550.08.1830114
GTGTTAG99100.08.1144261
CTTACAC86400.08.0745443
CTACACT103750.08.0728284
TAGGACT94400.08.0470744
Produced by FastQC (version 0.11.5)