FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842354

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842354
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64697567
Sequences flagged as poor quality0
Sequence length35-42
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG129550.011.2575335
CGTGCGA23250.010.68075110
TCTATAC87400.010.0484923
GTATAGG91150.09.8510971
GTATAGA105100.09.7761221
TACACTG142300.09.53817755
ATACTGT168700.09.4233386
CCTATAC88100.08.9818563
CCGTGCG52700.08.9530039
CTATACT138950.08.8870614
TAGACAG171300.08.6795165
TAGACTG119300.08.6554845
GTGTAGG138750.08.5178891
GTATTAG129150.08.2716631
TAGACCG18050.08.2567845
TCGTTCG17200.08.25318123
CTAACAC149000.08.1566443
CTACACT142050.08.1560424
CTAGCCT144850.08.1453674
TAGGACC72250.08.1037044
Produced by FastQC (version 0.11.5)