FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842355

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842355
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64697567
Sequences flagged as poor quality0
Sequence length35-42
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG133100.011.8494035
CGTGCGA26000.010.57606510
GTATAGA98500.010.2142921
ATACTGT168400.09.6710836
CCTATAC85650.09.0515063
GTATAGG91300.08.9985321
TCTATAC91500.08.8799633
CTACACT140650.08.8293094
TAGACTG118050.08.8065215
TACACTG138800.08.7681395
CTAGCCT152150.08.6400194
TAGACAG165600.08.6239925
GTATTAG134000.08.6206031
CCGTGCG55950.08.5929129
TCGTTGT28700.08.59046234
CTAGACT111600.08.4251994
TCTAGAC93500.08.2725933
GTGTAGA123050.08.2485171
TACACCG30750.08.19249255
GTACTAG46800.08.1897251
Produced by FastQC (version 0.11.5)